Neonatal screening for hypothyroidism by time - Resolved fluoroimmunoassay in Jharkhand

  • Rameshwar Prasad
  • Amar Verma
  • Anita Verma


Objective: To screen newborns for congenital hypothyroidism by time resolved fluoroimmunoassay (TRFIA) (TRFIA meter by
Perkin Elmer). Methods: Participants were randomly selected from the Department of Paediatrics/Obstetrics and Gynaecology,
Rajendra Institute of Medical Sciences and Maternity Hospitals around 20 km of Rajendra Institute of Medical Sciences, Ranchi.
Blood samples were obtained by heel prick for thyroid-stimulating hormone (TSH) estimation from newborns selected from abovementioned
hospitals. Samples were collected from 3rd day of life to 7th day of life on a filter paper card and analyzed by TRFIAbased
method. Results: 60% of the newborns were male and 40% were female among 150 newborns selected for study. Out of
them, 65.33% were term, 28% were pre-term, and 6.67% were post-term. 92% newborns had TSH level <10 mIU/L, 8% had TSH
between 10 and 20 mIU/L and none had TSH value >20 mIU/L. Conclusion: The prevalence of borderline TSH assay was 80/1000
newborns, which is significant. Screening studies for such conditions need larger sample size, stronger logistics to cover a larger

Keywords: Congenital hypothyroidism, Newborn, Thyroid-stimulating hormone, Time resolved fluoroimmunoassay
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How to Cite
Prasad, R., Verma, A., & Verma, A. (2017). Neonatal screening for hypothyroidism by time - Resolved fluoroimmunoassay in Jharkhand. Indian Journal of Child Health, 4(4), 542-544. Retrieved from
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